Zoledronic acid targets a nuclear NFATc2 protein stabilization pathway to suppress cancer growth

Introduction: Zoledronic acid, a widely used amino-bisphosphonate, has elicited significant attention due to its potential antitumoral activity. However, the molecular mechanisms underlying the strong growth suppressor functions of this antitumoral drug remains unclear. Aims: To analyze whether and how zoledronic acid suppresses tumor growth both in vitro and in vivo in pancreatic cancer. Methods: Using a pannel of pancreatic cancer cell lines, we examined the growth suppressor activity of zoledronic acid by 3H-thymidine incorporation and flow cytometry. Immunoblotting and ubiquitin assay were performed to deter…