Recombinant expression, purification and functional impact of CTGF/CCN2 and NOV/CCN3 on liver fibrogenesis
W Bohr, S Lux, E Borkham-Kamphorst, E Van de Leur, M Kupper, K Hoffmann, R Weiskirchen · Zeitschrift für Gastroenterologie · 2011 · Heft 01
Bibliografische Angaben
Zusammenfassung
Background: The CCN family of proteins, especially its prominent member, the Connective tissue growth factor (CTGF/CCN2/) has been identified as a possible biomarker for the diagnosis of fibrotic diseases [1]. As a downstream mediator of TGF-β1, it regulates expression and deposition of extracellular matrix proteins and promotes cell proliferation [2]. Another member of this family, the Nephroblastoma-overexpressed gene (NOV/CCN3), has growth-inhibiting properties. First reports further suggest that these two CCN proteins act opposite to each other [3]. Methods: We have established stable transfected HEK and Flp…
