Intermedin-treatment of pulmonary ischaemia/reperfusion injury in the living mouse

Background: The blood-air barrier of the lung is severely damaged by ischemia/reperfusion injury, resulting in leukocyte infiltration, decreased barrier function, inflammation and, consequently, in pulmonary oedema. Recently, we demonstrated that intermedin drastically decreases oedema formation in isolated ventilated and perfused mouse lungs. Here, we tested the hypothesis that intermedin protects mouse lungs from ischemia/reperfusion injury in vivo. Materials and Methods: C57BL/6 mice were anaesthetized, intubated, ventilated, and heparinized. The hilum of the left lung was dissected and clamped for 90min. The…