Effects of adipocytokines and free fatty acids on viability and apoptosis of rat INS-1E β-cells
R Spinnler, T Gorski, S Schuster, A Garten, S Laue, J Breitfeld, J Kratzsch, A Beck-Sickinger, A Körner, W Kiess · Diabetologie und Stoffwechsel · 2011 · Heft 05
Bibliografische Angaben
Zusammenfassung
Background and aims: The molecular interactions between adipose tissue and β-cells remain to be elucidated. Obesity is associated with a dysregulation of adipocyte secretory function. We asked, whether the adipocytokines, leptin, adiponectin, Nampt and vaspin, or the free fatty acids palmitate and oleate directly influence INS-1E β-cell survival and function. Methods: Changes in viability of INS-1E cells were detected by WST-1 assay. Adenylate kinase release was determined to assess cytotoxicity. Apoptosis was measured by Annexin V/PI Assay. For further characterization of activated apoptosis signaling pathways,…
