The role of the IL–17 axis in a mouse model of drug-induced liver injury

Objectives: Acetaminophen (APAP) overdose results in severe liver damage due to hepatocellular necrosis. Injury is triggered by release of pro-inflammatory cytokines and infiltration of immune cells. In this study, we investigated the involvement of the IL–17 axis during drug-induced liver damage. Methods: Primary hepatocytes (HCs) isolated from wildtype (WT), IL17A-, IL17A+F-, or IL23p19-deficient mice were exposed to different concentrations of APAP in vitro for 24hrs. Cell death was assessed by measuring LDH release. Moreover, APAP-induced hepatotoxicity was investigated in vivo by challenging WT, CCR6-, IL17…