Assessment of virotherapy resistance phenomena with subsequent evaluation of means helping to overcome such resistancies

Introduction: Measles vaccine virus (MeV) has shown to possess profound oncolytic capability. However, tumor resistances to MeV might endanger broad clinical success, a hypothesis which is underlined by our analysis of the NCI-60 tumor cell panel being infected with a suicide gene-armed vector (MeV ld-SCD); this SCD suicide gene locally converts the non-toxic prodrug 5-fluorocytosine (5-FC) into the cytotoxic chemotherapeutic agent 5-fluorouracil (5-FU), which is able to kill also neighbouring cells by diffusion („bystander effect”). Aim: We aimed at identifying mechanisms of resistance against the oncolytic vec…