Influence of Carbon Chain Length on Formation and Composition of Triglycerides in Free Fatty Acid- induced Steatosis

Background: Fatty liver, also called hepatic steatosis, is characterized by an imbalance between uptake, metabolism and excretion of free fatty acids (FFA). The resulting FFA-overload leads to the formation of triglyceride (TG)-filled lipid droplets in the cytoplasm of hepatocytes [1,2]. Our aim was to study the impact of FFA with different carbon chain length on the formation of TGs in hepatic steatosis. Further, we investigated how FFA-uptake and TG-metabolism is regulated by insulin and leptin in primary mouse hepatocytes. Methods: Hepatocytes were isolated from the C57BL/6N mice. For inducing steatosis we in…