Negative Selection In Vivo RNAi Screening Identifies a Ribosomal Protein As A New Therapeutic Target In Liver Cancer
M Pesic, K Wolter, T Wuestefeld, TW Kang, R Chawla, R Geffers, F Klawonn, P Schirmacher, N Malek, PK Premsrirut, T Longerich, L Zender · Zeitschrift für Gastroenterologie · 2014 · Heft 01
Bibliografische Angaben
Zusammenfassung
Due to the lack of treatment options hepatocellular carcinoma (HCC) represents the third most frequent cause of cancer related death. The major obstacle in HCC treatment is resistance to conventional chemotherapy and it becomes clear that new treatment strategies are urgently needed. We devised a system to conduct negative selection in vivo RNAi screens to identify new therapeutic target genes in Ras driven murine liver carcinomas. Multilocular invasive hepatocellular carcinomas develop after short latency when oncogenic Nras(G12V) is stably delivered into p19-/- mouse livers via transposable elements. As a proo…
