Xenotransplantation of cryopreserved/thawed human ovarian tissue in SCID mice: follicle development within 4 different observation periods

Fragestellung: How long should the xenotransplantation of cryopreserved/thawed human ovarian tissue be performed to evaluate the dynamics of follicular growth? Methodik: Cryopreserved/thawed human ovarian tissue was donated from 8 cancer patients. After rapid thawing tissue samples were cut into standardized pieces (3 mm diameter). Two tissue pieces were xenotransplanted into a subcutaneous neck pouch of 6-week-old ovarectomized SCID mice (n = 39) for 4, 8, 12 and 16 weeks. By the end of observation periods, grafts were recovered and processed for histomorphological analysis. Tissue directly after thawing served…