Visceral hyposensitivity induced by congenitally elevated gut permeability turns to hypersensitivity after stress in CA-MLCK mice
O Inczefi, H Eutamene, M Leveque, C Bétoulières, R Róka, A Rosztóczy, T Wittmann, J Turner, V Theodorou, L Ferrier · Zeitschrift für Gastroenterologie · 2014 · Heft 05
Bibliografische Angaben
Zusammenfassung
Background: Elevated gut permeability is linked to visceral hypersensitivity in patients with IBS. Using transgenic mice that express constitutively-active myosin light chain kinase (CA-MLCK) specifically within the intestinal epithelium in this way having gut hyperpermeability, we aimed to evaluate the pattern of visceral sensitivity and to determine putative mechanisms under basal and stress conditions. Materials and Methods: Adult CA-MLCK transgenic mice or WT littermates were used for experiments. To reveal gut permeability we used in vivo gavage method with fluorescein (400 Da). To measure visceral sensitiv…
