Systematic microrna expression pattern alterations in the colorectal adenoma-carcinoma sequence

Background: miRNA expression alterations can be observed in colorectal cancer (CRC), however dysregulation of miRNA might be present in various stages of precancerous lesions, such as adenoma. High-throughput screening platforms are available (microarray); and, RT-qPCR panels, which contain hundreds of miRNA specific oligos, are also provided. AIMS: Our primary aim was to identify the microRNA expression alterations between normal colonic tissue (N), tubular adenoma (ADT), tubulovillous adenoma (ADTV) and colorectal cancer (CRC) samples. Another purpose was to determine the level of miRNA by different methods an…