Experience from over 100 cases of preimplantation genetic analyses

Hypothesis: Preimplantation testing for single gene defects (PGD) and/or aneuploidy (PGS) provides good clinical benefit for couples with distinct genetic burden to fulfill their desire for a healthy child. Material and methods: Biopsies of 2 – 6 trophectoderm cells from day5-blastocysts were obtained by laser dissection. While embryos were vitrified, dissections were subjected to whole genome amplification (WGA) with multiple displacement (MDA) for PGD (REPLI-g Single Cell Kit, Qiagen, Hilden, Germany) or random priming for PGS (SurePlex Amplification System, BlueGnome Ltd., Cambridge, UK). PGD was performed by…