All-In-One: Advanced human parenchymal and non-parenchymal liver cell preparation
M Lutterbeck, K Kleinehr, S Driftmann, JW Treckmann, Z Mathé, G Kaiser, A Paul, K Skibbe, J Timm, A Canbay, G Gerken, JF Schlaak, R Broering · Zeitschrift für Gastroenterologie · 2015 · Heft 01
Bibliografische Angaben
Zusammenfassung
Introduction: Human liver cells are key players in liver health and disease. Liver cell preparations allow the investigation of diverse issues regarding liver physiology and pathophysiology. In particular, the quantity and quality of isolated cells are critical factors to study their function. Also, the usage of primary cells obtained from the same donor is important for comparative studies when using human cells. Aim of the study was the isolation of high quantity and quality primary human hepatocytes (PHH), Kupffer cells (KC), liver sinusoidal endothelial cells (LSEC) and hepatic stellate cells (HSC) from a si…
