MiR-210 and miR-155 are Upregulated in CFBE Cells and Involved in Fe-S Protein Assembly via ISCU Downregulation as well as HO-1 Expression via BACH1.

Cystic fibrosis (CF) is an inherited lung disorder associated with chronic endobronchial inflammation. Hemeoxygenase-1 (HMOX1), an enzyme involved in heme degradation, is upregulated in inflammation and negatively regulated via the transcription factor BACH1. Here we studied the role of microRNAs (miRs) in the heme metabolism of cystic fibrosis bronchial epithelial cells (CFBE41o-), applying qRT-PCR, luciferase promoter assays, immunoblotting, and enzyme activity assays. In comparison to normal human bronchial epithelial cells (16HBE14o-) miR-210 and miR-155 were 4- to 5-fold upregulated in CFBE41o- cells. MiR-2…