Cofilin-1-mediated growth regulation in pancreatic cancer cells is associated with downregulation of Gli1 and increased activity of the MAL/SRF transcription factor complex
B Kaistha, S Kirchhoff, H Schmidt, M Lauth, R Singh, B Sipos, T Gress, M Buchholz · Zeitschrift für Gastroenterologie · 2015 · Heft 08
Bibliografische Angaben
Zusammenfassung
Introduction: We have previously described a multi-step high-content screening approach to identify novel functionally relevant target genes in pancreatic ductal adenocarcinoma (Buchholz et al., PLoS-One, in press). The results of these analyses predicted an unexpected and previously undocumented role for the small actin-binding protein cofilin-1 in growth control of pancreatic cancer cells. Methods: Multiple tissue arrays, RNAi, cell proliferation and viability assays, FACS analysis, Western blot, inducible shRNA clones, reporter gene assays. Results: Cofilin-1 is strongly overexpressed in human pancreatic duct…
