Differential activation of RhoA and c-SRC in experimental and human fibrosis
J Görtzen, J Bierwolf, S Klein, R Schierwagen, CP Strassburg, W Laleman, JM Pollok, RG Wells, J Trebicka · Zeitschrift für Gastroenterologie · 2015 · Heft 12
Bibliografische Angaben
Zusammenfassung
Introduction: RhoA orchestrates cytoskeleton formation, migration and mobility via c-SRC and mDia1 in different cells. RhoA/ROCK also plays a crucial role in hepatic stellate cells and hepatic fibrogenesis. Matrix stiffness promotes HSC activation via cytoskeleton modulation. The role of SRC and mDia1 in HSC, and the role of matrix stiffness, were investigated in this study. Methods: Liver fibrosis was induced in rats using BDL, TAA or CCl4 models. Samples from cirrhotic patients and controls were collected at liver transplantations and tumor resections. Western Blot using phospho-specific antibodies against act…
