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Shear-induced fibrillar-like supramolecule of plasma fibronectin: A new form of fibronectin with enhanced activity in platelet adhesion and aggregation

KC Huynh, H Nguyen, V Stoldt, RE Scharf · Zeitschrift für Gastroenterologie · 2015 · Heft 12

Dokument
1084761
CareLit-ID
Jahr
2015
Publikation
PDF
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Metadaten
DOI
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Bibliografische Angaben

Zeitschrift
Zeitschrift für Gastroenterologie
Autor:innen
KC Huynh, H Nguyen, V Stoldt, RE Scharf
Ausgabe
Heft 12 / 2015
Seiten
–
Erschienen: 2015-12-14 00:00:00
ISSN
0044-2771
DOI
10.1055/s-0035-1567966

Zusammenfassung

Background: We reported on the formation of fibronectin fibrils (FN fibrils) induced by shear rates. Aims: Here, we characterized the function of shear-induced FN fibrils in platelet adhesion and aggregation. Methods: For adhesion experiments, CMFDA labeled platelets were placed onto immobilized FN or FN fibrils (25 µg/ml) for 30 min at 37 °C. In parallel experiments, platelets resuspended in FN-depleted plasma were placed onto immobilized FN, collagen, or fibrinogen (10 µg/ml) in the presence of soluble FN (300 µg/ml) or FN fibrils (10 µg/ml). For aggregation experiments, FN (5, 10, 300 µg/ml) or FN fibrils (5,…

Schlagworte

Fibrils Aggregation Plasma Platelet Adhesion Platelets Experiments Resuspended Fn-Depleted Collagen Shear-Induced Fibronectin Zeitschrift für Gastroenterologie