Sequential analysis of circulating tumor cells on genome and protein level: potential regulation of the invasion marker CapG by PIK3CA
M Neumann, Y Decker, A Franken, S Schömer, H Schneck, T Fehm, W Weichert, V Endriss, H Neubauer, D Niederacher · Senologie - Zeitschrift für Mammadiagnostik und -therapie · 2016 · Heft 04
Bibliografische Angaben
Zusammenfassung
Overexpression of macrophage capping protein (CapG) results in increased invasion potential in cancer cells. PI3K driven signal-transduction could lead to an increased CapG expression. Here we show isolated single CTC characterization for the invasion marker CapG and PIK3CA mutations. Classification of CTCs was done according to CellSearchTM. CTCs were isolated from CellSearch-Cartridge using the CellCelector (ALS, Jena) and transferred onto glass slides for subsequent immune fluorescence detection of CapG. A CapG-Score was established with CapG-negative white blood cells (WBC, CapG-Score 0) and two breast cance…
