Comparison of Anyplex II HPV HR- and Hybrid Capture2 testing in a screening population (WOLPHSCREEN)

Introduction: WOLPHSCREEN (Wolfsburg primary HPV screening for better cervical cancer prevention) comprises 24,000 women aged 30+ years who attended for routine screening between 2006 and 2016. In July 2015 we tested samples with Anyplex-II-HPV-HR (Seegene Inc) for HPV genotypes to compare Anyplex with HC2 results as gold standard. Methods: Selected samples were originally tested in 2007 with Hybrid Capture2 (HC2). A first set of stored and fresh samples tested earlier showed that the 2007 samples were still of good quality. 271 samples were tested for HPV with Anyplex in 2015. Diagnoses are based on the maximum…