Bisindolylmaleimide reverses the inhibitory effects of substance P and phorbol ester on pancreatic ductal bicarbonate secretion in the guinea pig

The stimulatory pathways controlling pancreatic ductal HCO3- secretion are well described. However, only a few data are available concerning inhibitory mechanisms, which may play an important role in the physiological control of the pancreas. We recently demonstrated that Substance P (SP) and Phorbol 12, 13-dibutyrate (PDBu) reduce pancreatic HCO3- secretion by inhibiting an apical Cl-/HCO3- exchanger on the duct cell. The aim of this study was to investigate a role of protein kinase C (PKC) in the action of SP. Methods: Small intra/interlobular pancreatic ducts were isolated from guinea pigs. The rate of HCO3-…