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Identification of proteins differentially expressed upon neurotrophin receptor activation using DIGE and MALDI-MS

O Apostolov, B Sitek, K Pfeiffer, K Stühler, HE Meyer, A Eggert, A Schramm · Klinische Pädiatrie · 2004 · Heft 05

Dokument
1101097
CareLit-ID
Jahr
2004
Publikation
PDF
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Metadaten
DOI
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Bibliografische Angaben

Zeitschrift
Klinische Pädiatrie
Autor:innen
O Apostolov, B Sitek, K Pfeiffer, K Stühler, HE Meyer, A Eggert, A Schramm
Ausgabe
Heft 05 / 2004
Seiten
–
Erschienen: 2004-05-27 00:00:00
ISSN
0300-8630
DOI
10.1055/s-2004-828547

Zusammenfassung

Activation of the neurotrophin receptor TrkA by its ligand NGF mediates growth inhibition and differentiation of neuroblastoma cells, whereas TrkB/BDNF signaling promotes proliferation and therapy resistance. Nonetheless, TrkA and TrkB exhibit a high level of sequence homology and use overlapping signaling pathways. To identify novel effector molecules contributing to the characteristic phenotypes of TrkA- and TrkB-expressing neuroblastoma cells, we analyzed global protein expression in neuroblastoma SY5Y cells stably transfected with TrkA or TrkB, and activated by their specific ligands in a time course from 0–…

Schlagworte

Proteins Cells Neuroblastoma Differentially Expressed Receptor Activation Signaling Identified Identification Neurotrophin Identify Klinische Pädiatrie