Promotor-Hypermethylation Is Causing Functional Relevant Downregulation of Methylthioadenosine Phosphorylase (MTAP) Expression in Hepatocellular Carcinoma
C Hellerbrand, M Mühlbauer, M Schuierer, S Wallner, I Behrmann, F Bataille, J Schölmerich · Zeitschrift für Gastroenterologie · 2005 · Heft 01
Bibliografische Angaben
Zusammenfassung
Homozygous deletions of human chromosomal region 9p21 occur frequently in several kinds of cancer and are associated with the loss of the tumor suppressor genes p16 (INK4a) and p15 (INK4b). In the same chromosomal region the methyladenosine phosphorylase (MTAP) gene is localised and therefore, may also serve as a tumor suppressor gene. The aim of this study was to analyse MTAP mutations and expression patterns in hepatocarcinoma (HCC). Methods: MTAP mRNA and protein expression were analysed with real time PCR, western blotting and immunohistochemistry in a) primary human hepatocytes and 3 different hepatoma cell…
