Candidate genes upregulated in density dependent growth inhibition of lung cancer cells

Prognosis of lung cancer remains poor despite the recent development of new chemotherapeutic agents. Novel therapeutic strategies therefore need to be developed. The search for endogenous factors inhibiting tumor growth in a paracrine/autocrine fashion might result in a well tolerated adjuvant tumor therapy. In this study we intended to identify candidate genes for such inhibitors of tumor cell growth. Native and heat inactivated supernatants of confluent, slow growing H460 tumor cell cultures and of sparse (non confluent), fast growing H460 tumor cell cultures were tested in proliferation assays. We observed th…