Legionella pneumophila induced IL-8 expression in human pulmonary epithelium by MAP kinases, NF-kB and histone modifications
B Schmeck, N Dje P'Guessan, J Lorenz, B Opitz, B Brell, A Flieger, M Witzenrath, S Rosseau, N Suttorp, S Hippenstiel · Pneumologie · 2006 · Heft 02
Bibliografische Angaben
Zusammenfassung
Introduction: L. pneumophila induced severe pneumonia in humans due to the inhalation of Legionella-contaminated water droplets. In this study we tested the hypothesis that Legionella induced pro-inflammatory activation of pulmonary epithelial cells and analyzed involved signalling pathways. Methods: human lung epithelial cells (A549), Bioplex-assay, IL-8-ELISA, Western blot, reportergen-assay, chromatin-immunoprecipitation (ChIP). Results: L. pneumophila induced the expression of various chemo- and cytokines in A549 cells and activated MAPK pathways. Inhibition of p38 MAPK reduced the expression of IL-8, which…
