Metabolomics: Metabolome measurement in human plasma
T Bobbert, I Göhring, K Mai, B Kristina, A Krotzky, C Gareth, L Wilmitzer, A Pfeiffer, J Spranger · Diabetologie und Stoffwechsel · 2006 · Heft 06
Bibliografische Angaben
Zusammenfassung
Objectives: Metabolomics is a new approach to precisely phenotype individuals and identify potentially novel biomarkers. Although GC- and LC-MS/MS based metabolomics has been established to determine the metabolome in plants, these techniques have not yet been evaluated in human plasma. We therefore aimed to analyze the reproducibility of measurements under various conditions. Methods: The analytical variability was investigated by repeated measurement of EDTA-plasma samples. Stability of the metabolome was analyzed by investigating samples after different periods at room temperature until freezing. In addition…
