Differential effects of PDZ-Adapterprotein NHERF1, E3KARP and PDZK1 knockout on the regulation of NHE3 transport activity in native murine colonic epithelium
A Cinar, M Chen, B Riederer, B Hogema, H de Jonge, M Donowitz, EJ Weinman, O Kocher, U Seidler · Zeitschrift für Gastroenterologie · 2006 · Heft 09
Bibliografische Angaben
Zusammenfassung
Background & Aims: The PDZ-adapter proteins NHERF1, E3KARP and PDZK1 have all been shown to bind to the sodium hydrogen exchanger NHE3, and to be expressed in the brush border membrane (BBM) of murine intestine. In PS120 fibroblasts, NHERF1 or E3KARP coexpression with NHE3 is required for cAMP-mediated inhibition of NHE3 trans-port activity. We therefore investigated, whether the three NHE3 binding PDZ-proteins show functional redundancy, or have differential roles in the native intestinal epithelium. Methods: NHERF1, E3KARP, and PDZK1deficient mice and their wild type NHE3 transport rates were assessed fluorome…
