Metabolic signature of breast cancer cell line MCF–7: Profiling of modified nucleosides via LC-IT MS coupling

Cancer is accompanied with metabolic disorders and shows characteristic effects on the activity of modifiying enzymes and DNA/RNA modifications, resulting in elevated amounts of excreted modified nucleosides. To understand the impaired RNA metabolism in breast cancer, we screened these metabolites the cell culture supernatants of the breast cancer cell line MCF–7 and compared it to the human mammary epithelial cells MCF–10A. The nucleosides were isolated and analyzed via 2D-chromatographic techniques: In the first dimension by cis-diol specific boronate affinity chromatography and subsequently by reversed phase…