Gene expression of the Toll-like receptor 9 and related intracellular signaling cascade in response to DNA from commensal bacteria and immunostimulatory DNA sequence by polarized…

Rationale: The aim of this study was to explore the effect of probiotic DNA on gene expression of toll-like receptor 9 (TLR9) and TLR9-related intracellular signalling cascade by the polarized intestinal epithelial cells (IECs). Methods: Polarized HT-29 cells were stimulated with TNFα in the presence or absence of DNA from Lactobacillus rhamnosus GG and Bifidobacterium longum, calf thymus DNA, CpG-B ODN and ODN backbone. TLR9 mRNA was assessed by TaqMan RT-PCR. Expression of TLR9 protein, degradation of inhibitor of kappa B alpha (IkBα) and p38 mitogen-activated protein (MAP) kinase phosphorylation were studied…