CareLit Fachartikel
Toll-like receptor activated hepatic stellate cells are potent regulators of hepatitis C virus replication
B Wang · Zeitschrift für Gastroenterologie · 2009 · Heft 01
Dokument
1104278
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Jahr
2009
Publikation
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Metadaten
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Zusammenfassung
Aims: While hepatic stellate cells (HSC) are known to be key mediators of liver fibrosis, only little is known about their role in the innate immune system of the liver., Methods: To address this question, murine HSCs were isolated from livers of C57BL/6J mice and human HSCs were isolated from liver samples obtained from resections. HSC were stimulated with TLR1–9 ligands for 20h. Expression of TNF-α, IFN-α, IFN-β and interferon stimulated genes (ISG) was assessed by qrt-PCR. Supernatants were harvested and assayed for antiviral factors in virus protection assays (EMCV) and human or murine HCV-replicon cell line…
Schlagworte
Toll like receptor
Hepatic stellate cell
liver fibrosis
Hepatitis C virus
Cytokines
Hepatitis
Cytokine
Address
Zeitschrift für Gastroenterologie
