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Hepatitis C virus (HCV) RNA quantitation in small-volume whole blood samples

T Bruns, K Steinmetzer, E Ermantraut, A Stallmach · Zeitschrift für Gastroenterologie · 2009 · Heft 01

Dokument
1104289
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Jahr
2009
Publikation
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Metadaten
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Bibliografische Angaben

Zeitschrift
Zeitschrift für Gastroenterologie
Autor:innen
T Bruns, K Steinmetzer, E Ermantraut, A Stallmach
Ausgabe
Heft 01 / 2009
Seiten
–
Erschienen: 2009-01-20 00:00:00
ISSN
0044-2771
DOI
10.1055/s-0029-1191927

Zusammenfassung

Since quantitation of HCV RNA in plasma or serum samples is a time and cost expensive procedure performed in central laboratories, we developed a hepatitis C RNA quantitation method for small-volume whole blood samples appropriate to capillary point-of-care diagnostic. HCV RNA was extracted out of 222 clinical 10µl whole blood and plasma samples of 82 chronically HCV-infected patients sample by capture hybridization to a single 25mer sequence specific Locked Nucleic Acid (LNA) modified 2rsquor;-O-Methyl RNA capture oligonucleotide, quantified by qRT-PCR and results were compared with COBAS Ampliprep/COBAS TaqMan…

Schlagworte

HCV LNA RT-PCR capillary diagnostic hepatitis C viral load whole blood Blood Hepatitis Zeitschrift für Gastroenterologie