Analysis of CD8+ T cell-mediated inhibition of hepatitis C virus replication using a novel immunological model
J Jo, U Aichele, N Kersting, R Klein, P Aichele, E Bisse, AK Sewell, HE Blum, R Bartenschlager, V Lohmann, R Thimme · Zeitschrift für Gastroenterologie · 2009 · Heft 01
Bibliografische Angaben
Zusammenfassung
While virus-specific CD8+ T cells are required for the control of hepatitis C virus (HCV) infection, the extent to which different effector functions contribute to the inhibition of viral replication has not been well defined. To address this important issue, we developed a novel immunological model by stably transducing the HLA-A2 gene into the replicon system, matching the epitope sequence of the replicon to the sequence targeted by an HCV-specific CD8+ T cell clone, and using luciferase activity to quantitate HCV RNA replication. Our findings demonstrate that HCV-specific CD8+ T cells strongly inhibit viral r…
