Screening Herbal Medicinal Products for CYP P450 enzyme inhibition with the fast, robust Supersome assay

The Supersome assay has long been used for industrial purposes to identify possible in vitro drug-drug interactions. Many of these interactions occur via induction or inhibition of the cytochrome P450 (CYP P450) enzyme system. The CYP P450 are a superfamily of membrane-bound, haeme-thiolate proteins, which are principally responsible for phase 1 oxidative metabolism and detoxification of xenobiotics and endobiotics (e.g. steroids, fatty acids, retinoids, eicosanoids, vitamins, cholesterol and prostaglandins). We have optimised and assessed the use of this assay for identifying possible herbal medicinal product (…