Submucous rather than myenteric neurons are activated by supernatants of mucosal biopsies from IBS patients

Introduction: From our previous study we know that incubation supernatants of colonic mucosal biopsies from patients with Irritable Bowel Syndrome (IBS) activate neurons of the human submucous plexus. Objectives: We used an animal model in guinea pigs to clarify whether the nerve sensitizing effect of IBS supernatants is also obvious in myenteric neurons. We therefore studied the effects of supernatants released from mucosal biopsies of 6 IBS patients (3 C-IBS and 3 D-IBS) and 4 healthy control persons (HC). Methods: A fast neuroimaging technique with a voltage sensitive dye (Di-8-ANEPPS) was used to record sing…