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Functional analysis of IGFBP-2 overexpression in mouse liver myofibroblasts

R Pannem, A Hoeflich, E Wolf, JG Scharf · Zeitschrift für Gastroenterologie · 2009 · Heft 09

Dokument
1105387
CareLit-ID
Jahr
2009
Publikation
PDF
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Metadaten
DOI
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Bibliografische Angaben

Zeitschrift
Zeitschrift für Gastroenterologie
Autor:innen
R Pannem, A Hoeflich, E Wolf, JG Scharf
Ausgabe
Heft 09 / 2009
Seiten
–
Erschienen: 2009-09-28 00:00:00
ISSN
0044-2771
DOI
10.1055/s-0029-1241577

Zusammenfassung

Background/aims: In liver cirrhosis hepatic expression and circulating levels of IGFBP-2 are significantly increased. However, the role of IGFBP-2 in liver fibrogenesis is unclear. Therefore, liver myofibroblats (LMFs) were isolated from livers of wild type (wt) and IGFBP-2 transgenic (IGFBP-2 (±)) mice and used to study the role of IGFBP-2 in cellular functions of mLMFs. Methods: LMFs were obtained by outgrowth from primary hepatocytes isolated from wt or CMV-IGFBP-2 transgenic mice. Expression of IGF-IR, IGF-II/M6-PR, IGFBP-2 and -3 mRNA was investigated by Northern blot hybridization. IGF-IRβ protein expressi…

Schlagworte

Igfbp-2 Mlmfs Expression Liver Increased Igf-I Synthesis Fibulin-2 Fibronectin-1 Fibrogenesis Isolated Transgenic Zeitschrift für Gastroenterologie