CareLit Fachartikel
Caspase 8-induced endosomal acidification is an upstream event in CD95-dependent hepatocyte apoptosis
R Reinehr, S Becker, A Sommerfeld, D Häussinger · Zeitschrift für Gastroenterologie · 2008 · Heft 01
Dokument
1106889
CareLit-ID
Jahr
2008
Publikation
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Zusammenfassung
Introduction: In hepatocytes, almost all CD95 receptor is located inside the cell. CD95 ligand was shown to trigger a NADPH oxidase-derived ROS-generation through a ceramide-dependent pathway which finally leads to CD95 trafficking to the plasma membrane [1]. Methods: In hepatocytes from rats and p47phox knock-out mice ceramide formation was detected by HPTLC, ROS-generation by DCFDA- and [Cl-] by MQAE-fluorescence and vesicular pH using endocytosed FITC-dextran. Acidic sphingomyelinase (ASM) knock-down was achieved using antisense oligonucleotides. Activation of the CD95 system was measured by CD95 immunoprecip…
Schlagworte
CD95
apoptosis
parenchymal cells
signaling
Ceramide
Caspase 8
After
Association
ATPase
Zeitschrift für Gastroenterologie
