CareLit Fachartikel
Intrahepatic expansion and maturation of telomerase-immortalized human fetal hepatocytes
H Wege, M Lüthgehetmann, T Volz, AW Lohse, M Dandri, J Petersen · Zeitschrift für Gastroenterologie · 2008 · Heft 01
Dokument
1106893
CareLit-ID
Jahr
2008
Publikation
PDF
nein
Metadaten
DOI
ja
zitierfähig
Bibliografische Angaben
Zusammenfassung
Aims: Telomerase reconstitution permits the unrestricted expansion of human fetal hepatocytes in vitro. As expected, the enhanced proliferative capacity of telomerase-immortalized hepatocytes is associated with an undifferentiated phenotype. In this study, we investigated the kinetic of intrahepatic expansion and maturation of immortalized human cells in a murine liver repopulation model. Methods: Scid/beige uPA mice (n=27) were intrasplenically transplanted with 2×106 immortalized human cells at population doubling 80 to 100. Engraftment and intrahepatic expansion were monitored by human-specific PCR for beta-g…
Schlagworte
HNF4alpha
gene expression
hepatocyte transplantation
liver repopulation
telomerase
After
Transplantation
Beta-Globin
Population
Zeitschrift für Gastroenterologie
