The influence of insulin and glucose on the expression of human insulin degrading enzyme

Aims: Insulin-degrading enzyme (IDE) is thought to play a key role in insulin degradation, and the liver is the main site of insulin clearance. Type 2 diabetes is characterized by decreased insulin clearance. IDE regulation in liver and fat cells is poorly studied, however in brain IDE was shown to be a downstream target of the insulin signaling cascade. The aim of this study was to investigate the IDE regulation by insulin and glucose in vitro in human hepatoma cell culture and in vivo in adipose tissue. Methods: Human hepatoma HepG2 cells were incubated in Dulbecco's modified Eagle's medium (DMEM) containing 1…