Transcription factor FBI-1 acts as a dual regulator in adipogenesis by coordinated regulation of cyclin-A and E2F-4
R Bilkovski, F Oberhäuser, A Droste, M Gomolka, U Leeser, M Udelhoven, W Krone, M Laudes · Diabetologie und Stoffwechsel · 2008 · Heft 04
Bibliografische Angaben
Zusammenfassung
Aims: Generation of new adipocytes plays a major role in the development of obesity. We previously have shown that transcriptional repressor FBI-1 exerts a dual effect in the process of adipogenesis by inhibiting proliferation and promoting differentiation of preadipocytes. The aim of the present study was to identify FBI-1 regulated molecular effectors which could account for these effects. Methods: In order to investigate the effect of FBI-1 on human cyclin A and E2F-4 promoter activity, dual-luciferase assays as well as EMSA and ChIP assays were performed in pEGFP-FBI-1 overexpressing and control cells. For e…
