Characteristic glycosylation patterns of human immune cells in NOD/SCID IL2Rγnull mice

Modifications in glycosylation occur during regular haematopoietic cell development and reflect their differentiation and activation status. Our aim is the characterization of the glycosylation of human immune cells in different immunologic organs during their development. The NOD/SCID IL2Rγnull mouse model is suitable to study these aspects of human hematopoiesis in vivo. Using plant lectins in combination with recombinant extracellular domains of sialic acid binding Ig-like lectins (siglecs) we determined the changes in glycosylation profiles of human lymphocytes isolated from bone marrow, blood, spleen and th…