CareLit Fachartikel

Chemotherapy resistance in pancreatic cancer – potential role of cancer stem cells

A Renner, Y Zhao, I Ischenko, P Camaj, JW Ellwart, H Blum, KW Jauch, CJ Bruns · Zeitschrift für Gastroenterologie · 2010 · Heft 08

Dokument
1108536
CareLit-ID
Jahr
2010
Publikation
PDF
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Metadaten
DOI
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Bibliografische Angaben

Zeitschrift
Zeitschrift für Gastroenterologie
Autor:innen
A Renner, Y Zhao, I Ischenko, P Camaj, JW Ellwart, H Blum, KW Jauch, CJ Bruns
Ausgabe
Heft 08 / 2010
Seiten
–
Erschienen: 2010-08-03 00:00:00
ISSN
0044-2771
DOI
10.1055/s-0030-1263977

Zusammenfassung

Aims: The presence of cancer stem cells in chemotherapy-resistant pancreatic cancer has not been analysed in depth. In the present study, we aimed to identify and characterize putative cancer stem cells (CSC) within human pancreatic cancer cell lines and chemotherapy-resistant variants. Methods: Pancreatic CSC were identified and characterized by flow cytometry, using Hoechst 33342 dye staining. CSC were isolated by high-speed flow cell sorting (MoFlo) and RNA was further analyzed by microarray hybridization, proteins by Western blotting. Isolated CSC as well as non-tumorigenic cancer cells were orthotopically x…

Schlagworte

Population Lead Zeitschrift für Gastroenterologie