CareLit Fachartikel
RNA stability in human liver: effects of different processing times, temperatures and storage methods
SML Lee, S Gashi, S Fröba, KW Jauch, WE Thasler · Zeitschrift für Gastroenterologie · 2011 · Heft 01
Dokument
1108794
CareLit-ID
Jahr
2011
Publikation
PDF
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Metadaten
DOI
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Bibliografische Angaben
Zusammenfassung
The Grosshadern Hospital Tissue Bank is a repository of clinically annotated surgical remnant tissues that can be accessed by researchers for basic and translational research. An important technology for elucidating molecular mechanisms of diseases is reverse transcription polymerase chain reaction (RT-PCR). However, accuracy of information garnered by RT-PCR is highly dependent on tissue quality. Thus, this study aimed to determine if tissues with longer processing times are suitable for RT-PCR studies and if processing temperatures or storage in RNAlater would help maintain RNA quality. Liver samples were coll…
Schlagworte
RNA stability
RT-PCR
gene expression
tissue bank
After
Blood
Zeitschrift für Gastroenterologie
