Hepatic differentiation of human embryonic stem cells in a 3D bioreactor environment
T Urbaniak, J Jensen, F Knöspel, M Lübberstedt, P Bjorquist, JC Gerlach, K Zeilinger · Zeitschrift für Gastroenterologie · 2011 · Heft 01
Bibliografische Angaben
Zusammenfassung
A number of articles addressing the hepatic differentiation of human embryonic stem cells (hESC) in conventional 2D culture systems have been published recently. However, the cell numbers and purity available through this culture method are limited. This study aims to investigate the hepatic differentiation of hESC in a dynamic 3D culture environment that supports cell differentiation and three-dimensional tissue reorganisation in vitro. Based on a perfused 3D-multicompartment bioreactor technology successfully used for primary human liver cell culture, a downscaled system (0.5ml cell volume) was developed to in…
