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In acute lymphoblastic leukaemia stemness is frequent and ubiquitous

K Rehe, K Wilson, S Bomken, M Stanulla, O Heidenreich, J Vormoor · Klinische Pädiatrie · 2011 · Heft 05

Dokument
1109171
CareLit-ID
Jahr
2011
Publikation
PDF
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Metadaten
DOI
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Bibliografische Angaben

Zeitschrift
Klinische Pädiatrie
Autor:innen
K Rehe, K Wilson, S Bomken, M Stanulla, O Heidenreich, J Vormoor
Ausgabe
Heft 05 / 2011
Seiten
–
Erschienen: 2011-05-05 00:00:00
ISSN
0300-8630
DOI
10.1055/s-0031-1277087

Zusammenfassung

Introduction: In ALL conflicting results persist regarding the frequency and identity of leukaemia-initiating cells, which self-renew and reconstitute the full original phenotype. Methods: Unsorted cells and sorted fractions (CD10, CD20 and CD34 high and low expression) from 30 ALL's of different risk groups were transplanted intrafemorally into NSG mice. Engraftment of serial transplantations and limiting dilutions (10–10,000 cells/mouse) was analysed by flow cytometry. Expression of self-renewal associated genes was investigated by real time PCR in CD34 high and low sorted blasts. Results: Unsorted blasts and…

Schlagworte

Cells Expression Blasts Sorted Results Original Unsorted Fractions Serial Transplantations Limiting Self-Renewal Klinische Pädiatrie