Comparison of two size variants of a miniaturized multi-compartment three-dimensional (3D)-bioreactor system for primary human hepatocyte cultivation
EC Wönne, U Müller-Vieira, J Frühwald, M Lübberstedt, S Hoffmann, F Knöspel, G Damm, AK Nüssler, K Zeilinger · Zeitschrift für Gastroenterologie · 2012 · Heft 01
Bibliografische Angaben
Zusammenfassung
Co-cultivation of primary human hepatocytes (pHH) with nonparenchymal liver cells in a perfused 3D-membrane capillary bioreactor (BR) enables more efficient long-term cell cultivation with maintenance of the physiological cell functions in comparison to standard 2D culture systems. Due to the restricted availability of primary human liver cells, miniaturization of the culture system while providing sufficient material for extra- and intracellular analyses is desirable for in vitro studies on hepatocyte physiology and metabolism. In this study two size variants of a miniaturized multi-compartment BR technology co…
