Next generation sequencing of the Ago2 interacting transcriptome identified chemokine family members as novel targets of neuronal microRNAs in hepatic stellate cells
A Noetel, N Elfimova, D Becker, P Nürnberg, A Teufel, HE Wasmuth, HP Dienes, M Odenthal · Zeitschrift für Gastroenterologie · 2013 · Heft 01
Bibliografische Angaben
Zusammenfassung
Background: After myofibroblastic transdifferentiation hepatic stellate cells (HSC), mainly involved in liver fibrosis by extracellular matrix production, exhibit an altered growth factor profile including increased expression of neuronal mediators. Here, we analyzed putative targets of neuronal microRNAs miR-9, miR-125b, and miR-128 by deep sequencing of the transcript population, interacting with miRNA/Argonaute 2 (Ago2) complex in myofibroblastic HSC. Methods: MicroRNA expression was quantified by real-time PCR in primary HSC, isolated from rat or human liver. Myofibroblastic HSC were transfected either with…
