Sorafenib modulates tumor-associated macrophages and perpetuates natural killer cell functions
MF Sprinzl, F Reisinger, D Hartmann, A Weinmann, PR Galle, M Schuchmann, G Otto, H Friess, M Heikenwälder, U Protzer · Zeitschrift für Gastroenterologie · 2013 · Heft 01
Bibliografische Angaben
Zusammenfassung
Background: Macrophages (MΦ) shape the environment of hepatocellular carcinoma (HCC) and inhibit anti-cancer immune responses. We investigated if Sorafenib restores classical macrophage polarization and is capable to trigger tumor-directed NK cell responses. Methods: Experiments were conducted with Sorafenib (25mg/kg) treated C57BL/6 wild-type as well as HCC bearing lymphotoxin-α/β or HBV transgenic mice. Monocyte derived MΦ or tumor-associated macrophages (TAM) isolated from HCC tissue were treated with Sorafenib (0.07–5.0µg/ml) and were co-cultured with autologous NK cells. MΦ and NK cell activation was analyz…
