CareLit Fachartikel

Characterization of oncogenes on chromosome 21 identified by shRNA-based viability screening

L Stachorski, VR Thangapandi, D Reinhardt, JH Klusmann · Klinische Pädiatrie · 2013 · Heft 05

Dokument
1111571
CareLit-ID
Jahr
2013
Publikation
PDF
nein
Metadaten
DOI
ja
zitierfähig

Bibliografische Angaben

Zeitschrift
Klinische Pädiatrie
Autor:innen
L Stachorski, VR Thangapandi, D Reinhardt, JH Klusmann
Ausgabe
Heft 05 / 2013
Seiten
–
Erschienen: 2013-05-22 00:00:00
ISSN
0300-8630
DOI
10.1055/s-0033-1343627

Zusammenfassung

Children with trisomy 21 (Down syndrome, DS) are predisposed to develop acute megakaryoblastic leukemia (DS-AMKL). Hypothesizing that the presence of an extra copy of human chromosome 21 (hsa21) is a basal requirement for the onset of DS-AMKL, we conducted a shRNA-based viability screening. We identified 26 oncogenic candidates genes on hsa21 conferring a profound growth disadvantage in DS-AMKL cell lines upon knockdown. 20 out of 26 trisomy 21-specific oncogenes lay in the chromosomal region 21q22.1 – 21q22.3, whereas 11 (of 14 tested) are overexpressed in DS-AMKL as compared to non-DS-AMKL. A secondary screeni…

Schlagworte

Ds-Amkl Trisomy Oncogenes Screening Hsa21 21Q22 Different Cells Chromosome Identified Shrna-Based Viability Klinische Pädiatrie