Cell culture models of pediatric high-grade glioma are susceptible to Parvovirus H-1 induced cytotoxicity

Oncolytic virotherapy with Parvovirus H-1 (H-1PV) represents a new therapeutic approach currently investigated in a phase I/IIa clinical trial recruiting adult patients with recurrent glioblastoma. For pediatric high-grade glioma patients there is also an urgent need of new therapeutic options. Here we analyzed, whether H-1PV is able to infect, to transduce and to selectively eliminate cell culture models of diffuse intrinsic pons glioma (DIPG) and pediatric glioblastoma in vitro. Neurosphere cultures of DIPG (n = 4) and adherent cultures of pediatric glioblastoma (n = 4) underwent H-1PV infection either at a lo…