Modulating liver regeneration by miR-142-3P encapsulated in microparticles
S Kuhn, K Splith, I Kämmerer, C Hegewald, L Feldbrügge, J Schulte am Esch, S Jonas, SC Robson, M Schmelzle · Zeitschrift für Gastroenterologie · 2014 · Heft 08
Bibliografische Angaben
Zusammenfassung
Background and aims: Plasma microparticles (MP) have recently been suggested to contain microRNA (miR) and act as novel intercellular carriers. MiR-142 – 3 p is associated with inflammatory responses. We hypothesized that hematopoietic stem cells (HSC) might respond to partial hepatectomy by shedding of CD133+ MP with miR-142 – 3 p MP levels being modulated by ectonucleotidase surface profiles. Methods: 70% hepatectomy was performed in C57Bl/6 wild type, Cd39 null and Cd73 null mice. MP were purified from the plasma and supernatant by 2-step ultracentrifugation. Total RNA isolated from MP and miR were detected b…
