Post-translational control of carnosinase activitiy in diabetes
V Peters, B Lanthaler, A Amberger, T Fleming, E Forsberg, M Hecker, A Wagner, GF Hoffmann, P Nawroth, J Zschocke, CP Schmitt · Diabetologie und Stoffwechsel · 2015 · Heft 04
Bibliografische Angaben
Zusammenfassung
Background: Carnosinase 1 (CN1) contributes to diabetic nephropathy by cleaving histidine-dipeptides which scavenge reactive oxygen and carbonyl species and increase nitric oxide (NO) production. In diabetic mice renal CN1 is increased, the regulatory mechanisms are unknown. Methods: We analysed the in vitro and in vivo regulation of CN1 activity using recombinant and human CN1, and the db/db mouse model of diabetes. Results: Glucose, leptin and insulin did not modify recombinant and human CN1 activity in vitro, glucose did not alter renal CN1 activity of WT or db/db mice ex vivo. Reactive metabolite methylglyox…
